Correlation between the cystathionine-r-lyase (CES) and the severity of peptic ulcer disease

Xiong Chen, Ying-Chun Wan, Tao Guo, Can-Xia Xu, Fen Wang

Abstract

Background: The infection of Helicobacter pylori (H. pylori) is one of the most important causes of gastric ulcer disease. The role of hydrogen sulfide (H₂S) production in H. pylori-induced gastric ulcer disease. Aim: The expression of cystathionine-γ-lyase (CSE) was determined, and correlated with the severity of gastric ulcer disease. Methods: 108 patients were selected based on the determination of gastric ulcer and the infection of Helicobacter pylori (H. pylori), including 36 normal control, 36 patients with H. Pylori-negative gastric ulcer, and 36 patients with H. Pylori-positive gastric ulcer. RT-PCR determination was performed to determine the expression of CSE, NF-κB and IL-8. Results: The expression of CSE, NF-κB and IL-8 was higher in gastric ulcer group than control group (p < 0.05). Compared with the H.pylori-negative gastric ulcer, the expression of CSE, NF-κB and IL-8 was higher than H.pylori-positive gastric ulcer group (p < 0.05). For H.pylori-negative gastric ulcer group, the expression of CSE positively correlated with the expression of NF-κB (r=0.98, p < 0.05) and IL-8 (r=0.95, p 0.05). For H.pylori-positive gastric ulcer group, the expression of CSE also positively correlated with the expression of NF-κB (r=0.99, p < 0.05) and IL-8 (r=0.85, p < 0.05). Conclusion: The expression of CSE was positively correlated with the severity of gastric ulcer.

Keywords: Helicobacter pylori (H. pylori), gastric ulcer, hydrogen sulfide (H₂S), cystathionine-γ-lyase (CSE)

African Health sciences Vol 14 No. 1 March 2014