Ameliorative effects of Cnidoscolus aconitifolius on alloxan toxicity in Wistar rats

O.I Azeez, A.A Oyagbemi, M.O Oyeyemi, A.A Odetola

Abstract

Background: Diabetes has been associated with several complications occasioned by oxidative stress. Thus, in treatment of the condition, these complications must also be taken into consideration. This study evaluates the effect of Cnidoscolus aconitifolius complications of diabetes induced by alloxan, on haematology and sperm morphometry using the Wistar rats.

Methods: Diabetes was induced in 25 rats using alloxan. The diabetic rats were then divided into five groups B-F consisting of five rats per group. Groups C-E were administered with 100mg/kg, 500mg/kg and 1000mg/kg of ethanolic leaves extract of Cnidoscolus aconitifolius, respectively, for four weeks post treatment with alloxan, while group F received Chlorpropamide (Diabenes®, Pfizer). The diabetic rats in group B were not treated while group A served as the non diabetic control.

Result: Following treatment with alloxan, there was anaemia, thrombocytopenia and leucopenia, while the sperm count, motility and live/dead ratio were significantly reduced. Sperm morphological abnormalities and erythrocyte osmotic fragility also increased significantly. Following treatment of alloxan treated-rats with the extract, there were significant increases in the PCV, RBC, Hb, WBC, MCV and the platelet values. Erythrocyte osmotic fragility, sperm count, motility and live/dead ratio also improved significantly.

Conclusion: Cnidoscolus aconitifolius extract was found to ameliorate the effects of alloxan induced diabetes on the haematology but not on the abnormal sperm morphometry in rats.

Keywords: Haematological parameters, osmotic fragility, sperm morphometry, Cnidoscolus aconitifolius, chlorpropamide