Cytotoxicity testing of aqueous extract of bitter leaf (Vernonia amygdalina Del) and sniper 1000EC (2,3 dichlorovinyl dimethyl phosphate) using the Alium cepa test.

Jane Ogoamaka Okwuzu, Peter Odeiga, Olubunmi AdetoroOtubanjo, Oliver Chukwujekwu Ezechi

1. Clinical Sciences Division, Nigerian Institute of Medical Research, Yaba Lagos.
2. Department of cell biology and genetics, University of Lagos, Akoka, Yaba Lagos.
3. Department of Zoology, University of Lagos, Akoka, Yaba Lagos.

Abstract:
Background: The unrefined nature of the herbal preparations from Vernonia amygdalina(VA) and toxicity potentials of Sniper may both have severe consequences on the biochemical and genetic systems.

Objectives: To assess the microscopic and macroscopic effects of these substances.

Methods:VA leafs and Sniper were prepared and dissolved in distilled water to give different concentrations. Series of baseline tests were carried out to establish concentration range for root growth. Series of twelve onion bulbs of three per series was prepared, with a series of three onion bulbs serving as control. Chromosomal aberrations were statistically analysed using chi- squared test. Root bundle mean length was obtained after 96 hours and EC50
values at 95% confidence interval was determined from a plot of root length against sample concentrations using Microsoft Excel software.

Results: Total cytotoxic effect was induced by 2% sniper and 70% VA. EC50 for VA and sniper were 33.07 and 0.346 respectively. The two substances induced chromosomal aberrations and the effect was concentration dependent.

Conclusion: There are risks of these widely used substances for therapeutic and environmental purposes.
Keywords: Chromosomal aberrations, Sniper 1000EC, Vernonia amygdalina, toxicity.